解剖学报

›› 2012, Vol. 43 ›› Issue (6): 767-771.doi: 10.3969/j.issn.0529-1356.2012.06.009

• 细胞和分子生物学 • 上一篇    下一篇

建立卵泡刺激素受体单位点基因突变体的卵巢癌SKOV3细胞和裸鼠模型

姚济芬1; 俞利平2; 董小龙1; 卢玲芬1; 张晓光1; 王亚云1; 韦兰芳1; 王水英1; 黄荷凤 3*   

  1. 1. 杭州师范大学附属医院妇产科，杭州 310015; 2. 杭州师范大学实验动物科学实验室，杭州 310036; 3. 浙江大学医学院附属妇产科医院，杭州 310006
  • 收稿日期:2012-04-05 修回日期:2012-05-07 出版日期:2012-12-06
  • 通讯作者: 黄荷凤

Ovarian carcinoma SKOV3 cell line and nude mice model with transfecting unit point mutant gene in follicle stimulating hormone receptor 

  1. 1. Department Obstetrics and Gynecology, Affiliated Hospital, School of Medicine, Hangzhou Normal University, Hangzhou 310015, China; 2. Laboratory of Experimental Animal,School of Medicine, Hangzhou Normal University, Hangzhou 310036, China; BR>3. Affiliated Obstetrics and Gynecology Hospital, Faculty of Medicine, Zhejiang University, Hangzhou 310006, China BR>
  • Received:2012-04-05 Revised:2012-05-07 Online:2012-12-06
  • Contact: HUANG He-feng

摘要： 目的 建立转染卵泡刺激素受体（FSHR）307和680位点突变的上皮性卵巢癌SKOV3细胞系和裸鼠动物模型。方法 将人卵巢颗粒细胞（来自体外受精取卵时废弃的颗粒细胞），行原代培养后用于FSHR RNA提取；FSHR和FSHR307、680位点突变重组蛋白构建；将FSHR和FSHR307、680位点突变蛋白转染SKOV3细胞株；将处理后的3组SKOV3细胞接种到裸鼠皮下，8周后处死裸鼠，并取瘤组织验证。

关键词: 卵泡刺激素受体, 突变, 上皮性卵巢癌, SKOV3细胞系, 反转录-聚合酶链反应, 裸鼠

Abstract: Objective To establish an epithelial ovarian carcinoma SKOV3 cell line and nude mice models with transfecting mutant gene in FSHR 307 and 680 site in order to provide two experimental models for further study and treatment of epithelial ovarian carcinoma by follicle stimulating hormome(FSH). Methods FSHR RNA from the primary culture of granular cells in human ovary were extracted. Recombination protein about FSHR and mutant point in FSHR 307 and 680 site were constructed and transfected to SKOV3 cell line. SKOV3, SKOV3 cells with FSHR and mutant point in FSHR 307 and 680 site cells were inoculated subcutaneously in female nude mice to form solid tumors. Pathological examinations were performed after 8 weeks. Results An epithelial ovarian carcinoma SKOV3 cell line and nude mice models with transfecting mutant gene in FSHR 307 and 680 site were established. Conclusion Establishing an epithelial ovarian carcinoma with transfecting mutant gene in FSHR 307 and 680 site in vitro and in vivo offers two considerable models to explore and treat epithelial ovarian carcinoma by FSH.

Key words: Follicle stimulating hormone receptor, Mutation, Epithelial ovarian carcinoma, SKOV3 cell line, RT-PCR, Nude mouse

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